Voltage response

An experiment was carried out to assess the affinity of a sugar transporter protein in plant cells by measuring electric current (Km) in response to a range of substrate-associated voltages. Nine voltage levels, ranging from -160 to 0 mV, were tested (Voltage), and the experiment followed a randomized complete block design with two blocks corresponding to separate experimental occasions (Rep). For each combination, one observation was recorded per block (DUnit, reflecting individual measurement units within each replicate). This dataset enables analysis of how membrane voltage influences transporter activity, with blocking to account for any variation between experimental runs.

Usage

voltage

Format

A data frame with 5 variables: ID, Rep, DUnit, Voltage, Km.

ID

Factor. Unique identifier for each observation.

Rep

Factor. Block number, corresponding to the experimental occasion (replicate).

DUnit

Factor. Measurement unit within each replicate (used in place of actual plot or plant randomization).

Voltage

Integer. Membrane voltage applied (in millivolts, mV; values range from -160 to 0).

Km

Numeric. Measured electric current for the respective voltage (unit appropriate to current, e.g., microamperes).

Source

Welham, S. J., Gezan, S. A., Clark, S. J., and Mead, A. (2015) Statistical Methods in Biology: Design and analysis of experiments and regression

Examples

lm(log(Km) ~ Rep + Voltage, data = voltage)
#> 
#> Call:
#> lm(formula = log(Km) ~ Rep + Voltage, data = voltage)
#> 
#> Coefficients:
#> (Intercept)         Rep2      Voltage  
#>   -0.334739    -0.197888     0.006777  
#>