This experiment is a pilot study intended to calibrate a scientific procedure. A demethylation agent is applied to plants: the agent has the effect of converting methylated nucleotides to non-methylated form, causing epigenetic changes that lead to abnormal phenotypes such as stunting and deformation (Amoah et al., 2008). The pilot study aimed to investigate the relationship between dose and the resulting proportion of plants with a normal phenotype. Seed was treated with the demethylation agent at six doses, including a zero control dose. Plants were grown in trays, each tray sown with seeds treated with the same dose of agent and each dose was replicated in four trays: two with 60 plants, and two with 100 plants. The trays were arranged as a CRD.